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Home of the Hysteriaceae, Mytilinidiaceae & Gloniaceae
The Molecular Systematics of the Hysteriaceae, Mytilinidiaceae & Gloniaceae
Intent: This website is devoted to mycology, a branch of the biological sciences that is devoted to the study of Fungi, organisms that are members of the Eumycota. On the Tree of Life Project, see the Eumycota for an introduction to the fungi. A wonderful introduction to mycology can also be found at See Bryce Kendrick’s The Fifth Kingdom. My research program is specifically related to the taxonomy and molecular phylogenetics of a group of bitunicate ascomycetes, loosely referred to as “Hysteriaceous” ascomycetes, currently classified in or previously affiliated with the Hysteriaceae, Gloniaceae and Mytilinidiaceae (Eumycota, Dikarya, Ascomycota, Pezizomycotina, Dothideomycetes, Pleosporomycetidae). On the Tree of Life site, click on the and click Hysteriales on the Tree of Life for an introduction to the group of fungi I study. On this website, I have tried to assemble much of what is known of these fungi, including most of the pertinent literature since Henrico Tode’s first descriptions in the 1790s to the present day. In an effort to facilitate species identification, a number of dichotomous keys are presented to species within each family. These keys take into consideration taxonomic changes brought about by DNA and amino acid sequencing studies (Schoch et al. 2006; Boehm et al. 2009a, b; Mugambi & Huhndorf 2009), and attempt to provide a morphological basis for the many new relationships revealed by molecular data. Although the keys are based on those first presented by Zogg (1962), they considerably expand upon them to include a number of new species and genera described since the original publication (e.g., Darker 1963, Tilak & Kale 1968, Barr 1975, 1990a, Barr & Blackwell 1980, Amano 1983, Speer 1986, Pande & Rao 1991, van der Linde 1992, Kantvilas & Coppins 1997, Lorenzo & Messuti 1998, Messuti & Lorenzo 1997, 2003, 2007, Vasilyeva 2000, 2001, Chlebicki & Knudsen 2001, Checa et al. 2007, Boehm et al. 2009a, b, Mugambi & Huhndorf 2009). In addition to incorporating new species and genera, the revised keys also take into consideration variation in ascospore measurements as presented by different authors, and include widened distribution reports as well. I hope other workers find these revised and updated keys useful for arriving at a species identification – for that is the goal of this website. Additionally, for the description of various taxa, I have quoted past workers of the group directly, duly citing the references from where the quotes originated. This was done so as to provide for a number of different opinions as to the specific characteristics for each species. It is hoped that this website will be dynamic and will incorporate future taxonomic changes as they manifest in the literature, most importantly those that originate from the acquisition of new DNA and protein sequence data. The goal is to determine whether morphological features historically used in the delineation of higher taxa in the Hysteriaceae and Mytilinidiaceae are phylogenetically informative in the context of sequence-based phylogenies. This site is dedicated to those mycologists who furthered our understanding of this group of fungi, specifically Hans Zogg, Margaret E. Barr Bigelow, and Emil Müller.
Current Research: Research interests are centered on the molecular systematics of the Hysteriaceae, Mytilinidiaceae & Gloniaceae. The specific aim of my current research program is to sequence phylogenetically important genomic regions – e.g., the small (nuSSU) & large (nuLSU) ribosomal subunits, the transcription elongation factor (TEF1) & the RNA polymerase subunits I & II (RPB1 & 2) to: (1) investigate ordinal affiliations within the Dothideomycetes for each family; (2) verify the placement of genera within each family and assess their degree of monophyly; and (3) to determine which morphological features are phylogenetically significant and therefore useful for generic delineation. Essentially, I am interested in whether phylogenies based on molecular character states will prove to be concordant with previous morphology-based classifications. Future projects will address speciation within each of the terminal clades defined. Towards this end, in conjunction with a number of international collaborators, a large culture collection has been assembled based on single ascospore isolates, representing the USA, England, France, Italy, Luxembourg, Germany, Russia, Argentina, South Africa, Spain, Israel, Morocco, Columbia and Tasmania. This culture collection is being added to continuously, with living cultures deposited with the Centraalbureau voor Schimmelcultures (CBS), The Netherlands, and dried voucher herbarium specimens depostied with the US National Fungus Collections (BPI), Beltsville, MD.
Prior Research: Past research projects have included: (1) the elucidation of ultrastructural fungal karyotypes using transmission electron microscopy among the plant rust fungi & allies (Boehm & McLaughlin, 1990; Boehm et al., 1992; Boehm & Bushnell, 1992); (2) the use of pulse field gel electrophoresis to deduce electrohporetic karyotypes among isolates of the Banana Wilt Fungus, Fusarium oxysporum f.sp. cubense (Boehm et al., 1994; Kistler et al., 1995); (3) the use of microsatellite primers to investigate population structure among isolates of the Apple Scab Pathogen, Venturia inaequalis (Boehm et al., 2003), marine monogonot rotifers used in aquaculture (Boehm et al., 2000) and the Pistachio Blight Pathogen Botryosphaeria dothidea (Maet al., 2001); and (4) the development of species-specific molecular diagnostics, based on VNTR satellites for PCR & dot blot hybridization, that were capable of detecting and quantifying in planta the brown rot of stone fruit fungal pathogen Monilinia fructicola (Boehm et al., 2001). Together, these publications formed the basis for five author citations in the leading university-level mycology textbook, namely Introductory Mycology, Fourth Edition (1996), by C.J. Alexopoulos, C.W. Mims and M. Blackwell. Two were also cited in the leading fungal genetics textbook, Fungal Populations and Species, by John Burnett (2003).
(3) Boehm, E.W.A. and D.J. McLaughlin. 1991. An ultrastructural karyotype for the fungus Eocronartium muscicola using epifluorescence preselection of pachytene nuclei. Canadian Journal of Botany 69: 1309-1320.
(4) Boehm, E.W.A., Wenstrom, J.C., McLaughlin, D.J., Szabo, L.J., Roelfs, A.P. and W.R. Bushnell. 1992. An ultrastructural pachytene karyotype of the Wheat Stem Rust Fungus Puccinia graminis f.sp. tritici. Canadian Journal of Botany 70: 401-413.
(6) Backlund, J.E., Boehm, E.W.A., Szabo, L.J. and W.R. Bushnell. 1992. Genome size and chromosome number for the Wheat Stem Rust Fungus Puccinia graminis f.sp. tritici. Vortr. Pflanzenzuchtg. 24: 19-21.
(7) Boehm, E.W.A., Ploetz, R.C. and H.C. Kistler. 1994. Statistical analysis of electrophoretic karyotype variation among vegetative compatibility groups in the Banana Wilt Fungus Fusarium oxysporum f.sp. cubense. Molecular Plant Microbe Interactions 7: 19
(9) Ravid, T., Tietz, A., Khayat, M., Boehm, E.W.A. and E. Lubzens. 1999. Lipid accumulation in the ovaries of a marine shrimp Penaeus semisulcatus de Haan. Journal of Experimental Biology 202: 1819-1829.
(10) Boehm, E.W.A., Gibson, O. and E. Lubzens. 2000. Cloning and characterization of satellite DNA sequences from the commercially important marine monogonont rotifers Brachionus rotundiformis and B. plicatilis. Marine Biotechnology 2: 38-48.
(13) Boehm, E.W.A., Freeman, S., Shabi, E. and T.J. Michailides. 2003. Microsatellite primers indicate the presence of asexual populations of the apple scab fungus Venturia inaequalis in coastal Israeli apple orchards. Phytoparasitica 31: 236-251.
(14) Boehm E.W.A, Schoch, CL & Spatafora JW. 2009a. On the evolution of the Hysteriaceae and Mytilinidiaceae (Pleosporomycetidae, Dothideomycetes, Ascomycota) using four nuclear genes. Mycological Research 113(4): 461-479.
(15) Boehm E.W.A., Mugambi G., Miller A.N., Huhndorf S.M., Marincowitz S., Spatafora J.W. & Schoch C.L. 2009b. A phylogenetic reappraisal of the Hysteriaceae, Mytilinidiaceae & Gloniaceae, with keys to world species. Studies in Mycology 64: 49-83.